Xpert MTB/RIF roll-out


Background

Earlier and improved tuberculosis (TB) case detection - including smear-negative disease, often associated with HIV co-infection - as well as expanded capacity to diagnose multidrug-resistant tuberculosis (MDR-TB) are global priorities for TB control. Conventional laboratory methods are slow and cumbersome and novel technologies for rapid detection are therefore the focus of TB research and development. With support from NIH, the Foundation for Innovative New Diagnostics (FIND) has partnered with Cepheid, Inc. (Sunnyvale, CA) and the University of Medicine and Dentistry of New Jersey (UMDNJ, Newark, NY) to develop a TB-specific automated, cartridge-based nucleic amplification assay (Xpert MTB/RIF) based on the GeneXpert multi-disease platform, currently unique in its simplification of molecular testing with fully integrated and automated sample preparation, amplification and detection required for real-time polymerase chain reaction. Xpert MTB/RIF detects M. tuberculosis as well as rifampicin resistance-conferring mutations directly from sputum, in an assay providing results within two hours.

In October 2013, WHO issued updated policy guidance and recommendations:

Expert Group Meeting report on XPERT MTB/RIF 2013

Policy Update on Xpert MTB/RIF assay for the diagnosis of pulmonary and extrapulmonary TB in adult and children

Xpert MTB/RIF Implementation manual: technical and operational ‘how-to’; practical considerations

For more on this story:

WHO monitoring of Xpert MTB/RIF roll-out

Fact sheet on Xpert MTB/RIF - October 2013

Briefing note on TBXpert project - September 2013

Updates on Xpert MTB/RIF roll out