These Truenat training modules were developed as a collaboration between the United States Agency for International Development (USAID) and its Infectious Disease Detection and Surveillance project (IDDS) and the Stop TB Partnership, as part of the introducing New Tools Project (iNTP). The content is based on the Stop TB/USAID/GLI Practical Guide to Implementation of Truenat Tests for the Detection of TB and Rifampicin Resistance, together with content provided by Molbio Diagnostics (Module 3). The material was technically reviewed and endorsed by the Global Laboratory Initiative.